linf scales each sample by its largest feature, preserves zeros, and groups samples by supported dominant and subdominant features. Supply a nonnegative matrix with samples in rows and features in columns. Stop after normalization, fit a hierarchy, or assign new samples to a reference you have already fitted.
After installation, this small example shows what the support threshold changes. The seed makes the toy counts reproducible.
library(linf)
set.seed(1)
counts <- matrix(rpois(30, 5), nrow = 10,
dimnames = list(paste0("s", 1:10), c("A", "B", "C")))
M <- normalize.linf(counts)
dominant <- linf.dominant.features(M)
fit <- linf.csts(M, n0 = 4, low.freq.policy = "absorb")| Feature | Provisional samples | Assigned after absorption |
|---|---|---|
| A | 4 | 6 |
| B | 4 | 4 |
| C | 2 | 0 |
A and B each have four provisional samples and meet n0 = 4. C has two,
so its samples are reassigned to the retained feature with greatest
abundance: A finishes with six samples and B with four. Absorption does
not mean A was those samples’ original maximum. M alone is sufficient
when you only need normalized profiles; it has row maximum one here and
preserves zeros.
The CRAN release is 0.3.0; the source here is 0.3.1 in development. The first example works with either. The two new navigation/dataset guides and the latest identity corrections are available in the development version.
# Released package (two installed workflows)
install.packages("linf")
# Development package (four installed vignettes)
# install.packages("devtools")
devtools::install_github("pgajer/linf", build_vignettes = TRUE)After installing the development package, read the rendered guides locally:
help("linf", package = "linf")
vignette("function-guide", package = "linf")
vignette("example-datasets", package = "linf")The published website is updated separately and may lag the development source. Use installed help for the version you run.
- Prepare profiles:
filter.asv()filters counts;normalize.linf()scales rows above tolerance while leaving zero and below-tolerance rows unchanged. - Fit groups:
linf.dominant.features()names each maximum;linf.csts()adds a support threshold;refine.linf.csts()subdivides selected groups. - Inspect results:
summary()accounts for assigned, rare and unassigned samples.linf.landmarks()selects observed rows by a target feature’s value, including rows closest to its mean or median; it does not average profiles. - Reuse a fit:
transfer.dcsts()assigns query samples to a frozen hierarchy.
L-infinity normalization is neither unit-sum normalization nor a log-ratio transform. It leaves zeros intact. Interpret grouping with the chosen feature set, support thresholds, low-support policy and tie rule; deeper is not necessarily better. The function guide source explains those choices and gives an executable workflow with stopping points.
The figure below illustrates depth-1 dCSTs fitted under the absorb policy to a bundled set of 766 gut microbiome samples from the American Gut Project (AGP). After filtering, 763 samples and 307 taxa remain. With n₀ = 30, samples from provisional dominance sample sets below the support threshold are reassigned to the retained state for which they have the largest normalized abundance; no composite rare category is shown.
The subset was deliberately stratified to include every sample assigned to four selected uncommon dCSTs; the remaining slots are a seed-42 simple random sample from the eligible background. Phenotypes do not influence selection. The object is suitable for demonstrating the package workflow, but its phenotype frequencies, effect sizes, and p-values must not be interpreted as population estimates because inclusion probabilities differ by dCST.
The 763 filtered samples are assigned among seven retained dCSTs. The largest are Bacteroides (239 samples), Escherichia-Shigella (134), and Staphylococcus (102). In total, 159 samples from low-support provisional dominance sample sets are absorbed into retained states.
See vignette("linf-intro", package = "linf") for a reproducible
demonstration using the bundled data.
The development package includes four installed vignettes. The first two links open maintained sources on GitHub; the commands open rendered installed guides. CRAN 0.3.0 contains the two longer workflows only:
- Finding your way around linf —
task-oriented function catalog, matrix conventions, policies,
landmarks and frozen-reference transfer. Open locally with
vignette("function-guide", package = "linf"). - Example datasets and reproducible
workflows — choose among the five
bundled objects, align metadata, inspect edge cases and try a separate
reference/query example. Open with
vignette("example-datasets", package = "linf"). - Dominant Community State Types: From Normalization to a Gut Demonstration — normalization, dCST construction and descriptive exploration of the stratified AGP gut subset.
- dCSTs for Vaginal Microbiome Data — fitting and descriptive agreement with Valencia labels on samples from the classifier’s training source, not held-out validation.
The interactive embedding article is website-only. It is not an installed vignette.
browseVignettes("linf")If you use this package, please cite:
Gajer, P. & Ravel, J. (2025). A New Approach to Compositional Data Analysis using L∞-normalization with Applications to Vaginal Microbiome. arXiv preprint arXiv:2503.21543 [stat.CO]. doi: 10.48550/arXiv.2503.21543
BibTeX
@article{gajer2025linf,
title = {A New Approach to Compositional Data Analysis using
{$L^{\infty}$}-normalization with Applications to
Vaginal Microbiome},
author = {Gajer, Pawel and Ravel, Jacques},
year = {2025},
eprint = {2503.21543},
archivePrefix = {arXiv},
primaryClass = {stat.CO},
journal = {arXiv preprint arXiv:2503.21543},
doi = {10.48550/arXiv.2503.21543},
url = {https://arxiv.org/abs/2503.21543}
}MIT © 2025 Pawel Gajer. See LICENSE. Bundled-data sources and upstream terms are recorded in bundled-data provenance.